Standard [CURRENT]
DIN EN ISO 22119:2011-11
Microbiology of food and animal feeding stuffs - Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22119:2011); German version EN ISO 22119:2011
- German title
- Mikrobiologie von Lebensmitteln und Futtermitteln - Real-time-Polymerase-Kettenreaktion (PCR) zum Nachweis von pathogenen Mikroorganismen in Lebensmitteln - Allgemeine Anforderungen und Begriffe (ISO 22119:2011); Deutsche Fassung EN ISO 22119:2011
- Publication date
- 2011-11
- Information
-
To be withdrawn in 2025-01 and replaced by: DIN EN ISO 22174, edition:2025-01
- Original language
- German
- Pages
- 18
- Publication date
- 2011-11
- Information
-
To be withdrawn in 2025-01 and replaced by: DIN EN ISO 22174, edition:2025-01
- Original language
- German
- Pages
- 18
- DOI
- https://dx.doi.org/10.31030/1573813
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The standard is applicable to the detection of food-borne pathogens in foodstuffs, and isolates obtained from them, using the polymerase chain reaction (PCR). This standard describes requirements for the amplification and detection of nucleic acid sequences (DNA or RNA after reverse transcription) by real-time PCR. The minimum requirements laid down in this standard provide the basis for comparable and reproducible results in individual and between different laboratories. The standard has been prepared by Working Group "Polymerase-Kettenreaktion zum Nachweis von Mikroorganismen" ("Polymerase chain reaction for the detection of microorganisms") of Working Committee NA 057-01-06 AA "Mikrobiologische Lebensmitteluntersuchung einschließlich Schnellverfahren" ("Microbiological analysis of foodstuffs including rapid method") of NAL.