DIN EN ISO 18415:2017-09

This document gives general guidelines for the detection and identification of specified microorganisms in cosmetic products as well as for the detection and identification of other kinds of aerobic mesophilic non-specified microorganisms in cosmetic products. Microorganisms considered as specified in this document might differ from country to country according to national practices or regulations. Most of them considered as specified microorganisms include one or more of the following species: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans. In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which this document is applicable. Products considered to present a low microbiological risk include those with low water activity, hydro-alcoholic products, extreme pH values, etcetera. The method described in this document is based on the detection of microbial growth in a non-selective liquid medium (enrichment broth) suitable to detect microbial contamination, followed by isolation of microorganisms on non-selective agar media. Other methods can be appropriate depending on the level of detection required. In this document specific indications are given for identification of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans. Other microorganisms that grow under the conditions described in this document may be identified by using suitable tests according to a general scheme. Other standards (for example, ISO 18416, ISO 21150, ISO 22717, ISO 22718) may be appropriate. Because of the large variety of cosmetic products within this field of application, this method might not be suited in every detail to some products (for example, certain water-immiscible products). Other methods (for example, automated) can be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable. This document (EN ISO 18415:2017) has been prepared by Technical Committee ISO/TC 217 "Cosmetics" (secretariat: ISIRI, Iran) in collaboration with Technical Committee CEN/TC 392 "Cosmetics", the secretariat of which is held by AFNOR (France). The responsible German standardization committee is Working Committee NA 057-07-01 AA "Kosmetische Mittel" ("Cosmetics") at DIN Standards Committee Food and Agricultural Products (NAL).