DIN EN ISO 16387:2014-05

Ecotoxicological test systems are applied to obtain information about the effects of contaminants in soil and are proposed to complement conventional chemical analysis. ISO 15799 includes a list and short characterization of recommended and standardized test systems. Aquatic test systems with soil eluate are applied to obtain information about the fraction of contaminants potentially reaching the groundwater by the water path (retention function of soils), whereas terrestrial test systems are used to assess the habitat function of soils. For the latter, a standardized test system using Enchytraeidae (a chronic test with end-point reproduction) is proposed. This International Standard describes a method that is based on the determination of acute and sublethal effects of contaminated soils to adult Enchytraeidae of the genus Enchytraeus. Optionally, the method can be used for testing substances added to standard soils (for example, artificial soil) for their sublethal hazard potential to Enchytraeidae. This Technical Specification has been prepared by Technical Committee ISO/TC 190 "Soil quality". The responsible German Committee is NA 119-01-02-04 UA "Biologische Verfahren" ("Biological methods").