Standard [CURRENT]
DIN EN ISO 11701:2010-04
Vegetable fats and oils - Determination of phospholipids content in lecithins by HPLC using a light-scattering detector (ISO 11701:2009); German version EN ISO 11701:2009
- German title
- Pflanzliche Fette und Öle - Bestimmung des Gehaltes an Phospholipiden in Lecithinen durch HPLC mittels eines Lichtstreudetektors (ISO 11701:2009); Deutsche Fassung EN ISO 11701:2009
- Publication date
- 2010-04
- Original language
- German
- Pages
- 17
- Publication date
- 2010-04
- Original language
- German
- Pages
- 17
- DOI
- https://dx.doi.org/10.31030/1551114
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This standard specifies a method for the quantitative determination of phospholipids by high performance liquid chromatography (HPLC) using a diol column and a light-scattering detector. The method is applicable to crude, oil-containing lecithins and to oil-free lecithins and lecithin fractions from vegetable fats and oils. The method is not applicable to animal and ruminant lecithins and enzymatically hydrolyzed lecithins; the peak separation of lysophosphytidylethanolamine (LPE), lysophosphatidylinositol (LPI) and lysophosphatidic acid (LPA) is insufficient. The International Standard has been prepared by ISO/TC 34/SC 11 "Animal and vegetable fats and oils" (secretariat: BSI, United Kingdom). CEN/TC 307 "Oilseeds, vegetable and animal fats and oils and their by-products - Sampling and test methods" (secretariat: AFNOR, France) is responsible for the European Standard. On the national level "Gemeinschaftsausschuss für die Analytik von Fetten, Ölen, Fettprodukten, verwandten Stoffen und Rohstoffen" (GA Fett) ("Joint Committee for the analysis of fats, oil, fat products, related substances and raw materials") of German Society for Fat Science (DGF) and NAL are responsible for the standard.