DIN EN ISO 10710:2013-06

This International Standard specifies a method for the determination of the inhibition of growth of the macroalga Ceramium tenuicorne by substances and mixtures contained in seawater or by waste water with salinities between 4S and 32S. This method is applicable to substances that are easily soluble in water. With modifications as described in ISO 14442 and ISO 5667-16, the inhibitory effects of poorly soluble organic and inorganic materials, volatile compounds, metals, waste water, marine water samples, and elutriates of sediments can be tested. Algal tips from monocultures of ceramium female gametophytes are grown in defined test conditions and in a defined medium containing a range of concentrations of the test sample. The test solutions are incubated for a period of 7 d after which the increase in length is measured and the growth rate is calculated. The growth inhibition is determined as a reduction in growth rate, relative to control cultures grown under identical conditions. The red macroalga Ceramium tenuicorne belongs to Ceramiaceae, Rhodophyta. The species can be used as a model organism for the near coastal ecosystem. This species is found in temperate marine waters in both the northern and southern hemispheres and is thus relevant for large areas. As primary producers, they are a food source for many invertebrates and serve as living habitat for bacteria, invertebrates, and juvenile fish. They also serve as substrate for many oviparous fish species. The underlying International Standard has been prepared by ISO/TC 147/SC 5/WG 5 "Toxicity - Algae and aquatic plants" and has been adopted by Technical Committee CEN/TC 230 "Water analysis", the secretariat of which is held by DIN. The responsible German committee is NA 119-01-03-05-03 AK "Marine Biotests" ("Marine biotests") at NA 119-01-03 AA "Wasseruntersuchung" ("Water Analysis") at the NAW. The national revision of this project is funded by the Länderfinanzierungsprogramm "Wasser, Boden und Abfall" (federal funding programme "Water, soil and waste").