DIN EN ISO 10273:2017-08

After Campylobacter and Salmonella, Yersinia enterocolitica is the third most common bacterial pathogen registered in humans in Germany in connection with intestinal diseases. The majority of disease-causing Yersinia species in Germany are Yersinia enterocolitica strains with characteristic surface structures. Yersinia enterocolitica can cause acute cases of illness with watery diarrhea, fever, abdominal pain and vomiting after an incubation period of seven to ten days. This document is a revision of the standard DIN EN ISO 10273:2003-12 and DIN EN ISO 10273 Corrigendum 1:2007-05. The method has been brought up to the state of the art and validated in an interlaboratory test as part of a mandate from the EU Commission to CEN (M/381). This method applies to products intended for human consumption or as animal feed and to environmental samples in the area of food production and food handling. It serves to protect consumer health. Important aspects of this test procedure are as follows: - Direct plating on CIN agar and PSB and ITC enrichment at 25 °C are mandatory. - Cold enrichment is included as optional in an informative annex. - The plating of PSB and ITC via KOH treatment on CIN agar is mandatory. Plating on chromogenic agar is optional. The investigations carried out as part of the interlaboratory comparison have shown that some laboratories benefit from the use of chromogenic media. However, there was not enough data to justify this step as mandatory. As a result of the study results, the plating of PSB and ITC without KOH treatment is changed to an optional step; that is, only as information in the form of a note. The responsible committee for this standard is NA 057-01-06 AA "Mikrobiologie der Lebensmittelkette" ("Microbiology of the food chain") at DIN.