DIN EN 16158:2012-05

DIN EN 16158 specifies a high-performance liquid chromatographic (HPLC) method for the determination of the semduramicin content at authorized level in animal feeding stuffs, using mass spectrometry detection or post-column derivatization and UV detection. This method is applicable to poultry feed. The limit of quantitation is 1,0 mg/kg when mass spectrometry is used for detection and 3,0 mg/kg when the detection is performed by UV with post-column derivatization. Lower limits of quantitation are achievable but this is to be validated by the user. The method allows the discrimination of semduramicin from monensin, salinomycin, narasin, maduramicin and lasalocid. The European Standard has been prepared by CEN/TC 327 "Animal feeding stuffs - Methods of sampling and analysis", the secretariat of which is held by NEN (Netherlands). Collaboration of DIN for the field of responsibility of CEN/TC 327 is performed through the national mirror committee, Working Committee NA 057-03-03 AA "Futtermittel" ("Animal feeding stuffs") of NAL.