DIN EN 15911:2011-01

This standard describes a HPLC method with evaporative light scattering detection (HPLC-ELSD) for the simultaneous determination of nine sweeteners (acesulfame-K, alitame, aspartame, cyclamic acid, dulcin, neotame, neohesperidine dihydrochalcone, saccharin and sucralose) in foodstuffs. The method has been fully validated through collaborative trial, according to the IUPAC Harmonised Protocol, on analyte-matrix combinations of all nine sweeteners in beverages and canned or bottled fruits. The procedure involves extraction of the nine sweeteners with a buffer solution, sample clean-up using solidphase extraction cartridges followed by HPLC-ELSD analysis. The European Standard has been prepared by CEN/TC 275 "Food analysis - Horizontal methods" (secretariat: DIN) after in-depth preliminary work of WG 2 "Sweeteners". The responsible German standardization committee is Working Committee NA 057-01-11 AA "Süßungsmittel" ("Sweeteners") of NAL.