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DIN 10102:1988-06

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Standard [CURRENT]

DIN 10102:1988-06

Microbiological analysis of meat and meat products; detection of clostridium botulinum and botulinum toxin

German title: Mikrobiologische Untersuchung von Fleisch und Fleischerzeugnissen; Nachweis von Clostridium botulinum und Botulinum-Toxin
Publication date: 1988-06
Original language: German
Pages: 5

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Publication date: 1988-06
Original language: German
Pages: 5
DOI: https://dx.doi.org/10.31030/2244831

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Content

Content (de)

Anwendungsbereich und Zweck
Begriff
Kurzbeschreibung des Verfahrens
Chemikalien und Nährböden
- Modifiziertes Kochfleisch-Medium
  - Nährlösung
  - Herstellung des vollständigen Kochfleisch-Mediums
- Caseinpepton-Glucose-Hefeextrakt-Trypsin-Medium (TPGYT-Medium)
  - Grundnährlösung
  - Trypsin-Lösung
  - Vollständiges Nährmedium
- Eigelb-Agar nach McCIung und Toabe (AEY-Agar; Anaerobic Egg Yolk-Agar)
  - Grundnährboden
  - Eigelb-Emulsion
- Gelatine-Phosphat-Pufferlösung
  - Herstellung
- Ethanol,
- Vaseline-Paraffin-Gemisch (Vaspar)
- Trypsin (z. B. 1 :250)
- Seren
Geräte
- Mikroskop.
Probenahme
Durchführung
- Nachweis vermehrungsfähiger Clostridium botulinum-Zellen bzw. -Sporen
  - Vorbereitung der Probe (Homogenisierung)
  - Anreicherung
  - Isolierung
  - Bestätigung
- Nachweis von Botulinum-Toxin
  - Herstellung des Extrakts
  - Trypsinbehandlung
  - Hitzebehandlung
  - Intraperitoneale bzw. subkutane Injektion in Mäuse
  - Beobachtung der Versuchstiere und Auswertung
  - Typisierung des Toxins mit Seren
Untersuchungsbericht
Zitierte Normen und andere Unterlagen
Erläuterungen

ICS

07.100.30, 67.120.10

DOI

https://dx.doi.org/10.31030/2244831

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