DIN EN ISO 24276:2013-10

This document is an amendment to International Standard ISO 24276:2006, which describes how to use the standards for nucleic acid extraction (ISO 21571), qualitative nucleic acid analysis (ISO 21569), quantitative nucleic acid analysis (ISO 21570) and protein-based methods (ISO 21572), and explains their relationship in the analysis of genetically modified organisms in foodstuffs. It contains general definitions, requirements and guidelines regarding: laboratory set-up, method validation requirements, description of methods and test reports. This standard has been established for food matrices, but could also be applied to other matrices (for example, seeds, feed and plant samples from the environment). The following modifications are included: - reference to other standards in this series dealing with the analysis of GMOs in foodstuffs has been updated; - several definitions have been corrected and adapted to the definitions already specified in other standards; Figure 1 has been corrected by including a missing step in the process scheme; - information regarding LOD and LOQ have been more clearly described; - Footnote b to Table 1 in 5.2 has been supplemented by a description as to how the environmental controls can be executed; - requirements for expression of positive, negative and ambiguous results have been updated; - requirements for the test report have been supplemented; - Bibliography has been updated. The responsible international committee is ISO/TC 34/SC 16 "Horizontal methods for molecular biomarker analysis". The committee responsible for this standard is NA 057-01-04 AA "Gentechnisch modifizierte Lebensmittel" ("Genetically modified foodstuffs") at DIN.